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SARS-CoV-2 Spike Protein Total Antibody ELISA Kit– MSE Supplies LLC

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SARS-CoV-2 Spike Protein Total Antibody ELISA Kit

SKU: E-EL-E607

  • $ 48995
  • Save $ 8700



SARS-CoV-2 Spike Protein Total Antibody ELISA Kit

Product Details

Properties

Assay Type Sandwich-ELISA
Format 24T, 96T, 96T*5, 96T*10
Assay time 2.5h
Sample type serum and plasma
Sample volume 100 μL
Storage 2-8℃
Interpretation Qualitative
Application This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 Spike Protein Total Antibody in serum or plasma.
Reproducibility Both intra-CV and inter-CV are < 10%
Synonyms COVID 19 Spike Protein Total Antibody,Spike Protein Total Antibody,Spike Protein Antibody,SP Total Antibody,SP Antibody,SARS-CoV-2 Spike(RBD) Tab、2019-nCoV Spike(RBD) Tab、COVID-19 Spike(RBD) Tab


Test Principle

This ELISA kit uses the Sandwich-Ag ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with recombinant SARS-CoV-2 Spike Protein. After adding samples (or controls) to wells, the total antibodies against SARS-CoV-2 Spike Protein will combine with the pre-coated SARS-CoV-2 Spike Protein. After washing completely, add Horseradish Peroxidase (HRP) conjugated SARS-CoV-2 Spike Protein to develop the antigen-antibody-HRP conjugated antigen complex. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike Protein, antibodies against SARS-CoV-2 Spike Protein and HRP conjugated SARS-CoV-2 Spike Protein will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike Protein total antibody exists in the tested samples or not.

Kit Components and Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the conditions on the next page once the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips 2-8℃, 6 months
48T: 8 wells ×6 strips
Positive Control 96T: 2 vials
48T: 1 vial
Negative Control 96T: 2 vials
48T: 1 vial
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL -20℃ (Protect from light), 6 months
48T: 1 vial, 60 μL
Sample & Control Diluent 2 vial, 20 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer(25×) 1 vial, 30 mL
Stop Solution 1 vial, 10 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from
light) , 6 months
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

 

Assay Procedures

1. Add 100µL each pre-treated Samples and Controls. Incubate for 90 min at 37°C.

2. Aspirate and wash the plate for 3 times.

3. Add 100µL of HRP Conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times.

4. Add 90µL of Substrate Reagent. Incubate for about 15 min at 37°C.

5. Add 50µL of Stop Solution.

6. Read the plate at 450nm immediately. Calculation of the results.