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Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)
SKU: E-BC-K261-S-100
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Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)
| SKU # | E-BC-K261-M |
| Detection Instrument | Spectrophotometer (510 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Synonyms | TG |
| Sample type | Serum, plasma, tissue |
| Sensitivity | 0.19 mmol/L |
| Detection range | 0.19-8.0 mmol/L |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 50min |
| Precision | Average inter-assay CV: 6.700% | Average intra-assay CV: 2.400% |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of Sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.19-8.0 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| Human serum | 1 |
| Mouse serum | 1 |
| Rat plasma | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) for serum (plasma) samples; The diluent is anhydrous ethanol for tissue samples.
Detection Principle
The color depth of the generated quinones is directly proportional to the triglyceride content. The absorbance values of the standard tube and the sample tube are measured respectively, and the triglyceride content in the sample can be calculated.
Kit Components & Storage
| Item | Component | Size (96 T) | Storage |
| Reagent 1 | Enzyme Working Solution | 100 mL × 1 vial | 2-8℃, 12 months shading light |
| Reagent 2 | 2.26 mmol/L Glycerinum Standard | 0.1 mL × 1 vial | 2-8℃, 12 months shading light |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 0.66 | 2.50 | 6.40 |
| %CV | 2.7 | 2.3 | 2.2 |
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 0.66 | 2.50 | 6.40 |
| %CV | 6.2 | 6.8 | 7.1 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 94%.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Expected Conc. (mmol/L) | 0.85 | 3.4 | 7.2 |
| Observed Conc. (mmol/L) | 0.8 | 3.2 | 6.6 |
| Recovery rate (%) | 98 | 93 | 91 |
Sensitivity
The analytical sensitivity of the assay is 0.019 mmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.
Standard Curve
As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:
|
Concentration (mmol/L) |
0 | 1 | 2 | 4 | 5 | 6 | 8 |
| Average OD | 0.075 | 0.140 | 0.193 | 0.301 | 0.508 | 0.700 | 0.922 |
| Absoluted OD | 0.000 | 0.065 | 0.118 | 0.226 | 0.433 | 0.625 | 0.847 |