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Tyrosine Ammonia-lyase (TAL) Activity Assay Kit– MSE Supplies LLC

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Tyrosine Ammonia-lyase (TAL) Activity Assay Kit

SKU: E-BC-K851-M-96

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Tyrosine Ammonia-lyase (TAL) Activity Assay Kit

SKU # E-BC-K851-M
Detection Instrument Microplate reader (323-343 nm, optimum wavelength: 333 nm)
Detection method
Colorimetric method


Product Details

Properties

Synonyms TAL
Sample type Fruit juices, plant and animal tissue
Sensitivity 0.12 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 70 min
Other instruments required Micropipette, Incubator, Centrifuge, Water bath
Storage 2-8℃
Valid period 12 months


Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
20% Epipremnum aureum tissue homogenate 1
20% Celery tissue homogenate 1
20% Cilantro tissue homogenate 1
20% Rat liver tissue homogenate 1
20% Rat kidney tissue homogenate 1
20% Corn tissue homogenate 1

 

Note: The diluent is reagent 1.

 

Detection Principle

TAL can decompose tyrosine to produce 4-coumaric acid, which has a strong absorption peak at 333 nm. Therefore, the activity of TAL can be calculated by measuring the OD value at 333 nm.

 

Kit Components & Storage

Item Component Size 1(48 T) Size 2(96 T) Storage
Reagent 1 Extracting Solution 25 mL × 1 vial 50 mL × 1 vial 2-8℃, 12 months
Reagent 2 Buffer Solution 35 mL × 1 vial 35 mL × 2 vials 2-8℃, 12 months
Reagent 3 Substrate Power ×1 vial Power ×2 vials 2-8℃, 12 months
Reagent 4 Stop Solution 1.5 mL × 1 vial 1.5 mL × 2 vials 2-8℃, 12 months
UV Microplate 96 wells No requirement
Plate Sealer 2 pieces

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.


Sensitivity

The analytical sensitivity of the assay is 0.12 U/mL. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.