Uncoated Mouse POSTN/OSF-2(Periostin) ELISA Kit
SKU: E-UNEL-M0069
Uncoated Mouse POSTN/OSF-2(Periostin) ELISA Kit
Detection Range | 0.16-10 ng/mL |
Application
The new series of ElabscienceĀ® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.
For in vitro quantitative determination of POSTN/OSF-2 concentration in Mouse serum, plasma.
Properties
Assay type | Sandwich |
Format | 96T*5 |
Assay time | 24h |
Reactivity | Mouse |
Storage | -20ā. Please refer to the instructions for details. |
Sample type | serum, plasma |
Sample volume | 100Ī¼L |
Kit components
Item | Specifications | Storage |
---|---|---|
Micro ELISA pre-Plate(Dismountable) | 96T*5ļ¼5 plates, 96T | -20ā, 12 months |
Reference Standard | 96T*5ļ¼1 vial | |
Concentrated Capture Ab | 96T*5ļ¼1 vial 120Ī¼L | |
Concentrated Biotinylated Detection Ab | 96T*5ļ¼1 vial 120Ī¼L | |
Concentrated HRP Conjugate | 96T*5ļ¼1 vial 120Ī¼L | -20ā(Protect from light), 12 months |
Product Description | 1 copy | / |
Certificate of Analysis | 1 copy |
Test Principle
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1. Analyte-specific antibody (capture antibody) is bound to a pre-microplate, unbound capture antibody is washed away. Then plates are blocked. |
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2. Samples or standards are added and any analyte present is bound by the immobilized capture antibody. Unbound materials are decanted. |
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3. The added Biotinylated Detection Ab will bind to the analyte been captured by the immobilized antibody. Unbound Biotinylated Detection Ab is washed away. |
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4. HPR Conjugate is used to bind to the Biotinylated Detection Ab. Unbound HPR Conjugate is washed away. |
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5. TMB substrate solution is added to the wells and a blue color develops in proportion to the amount of analyte present in the sample. Then the Stop Solution is added to stop the color change of substrate, and the color inside the well changes from blue to yellow. The absorbance of the color at 450 nm is measured. |