Thank you!

Your quote has been successfully submitted!

For products requiring additional information, our team will contact you within 1 business day

Failed

There was an error submitting your quote. Please try again.

Vitamin E (VE) Colorimetric Assay Kit– MSE Supplies LLC

Free Shipping on MSE PRO Online Orders of $500 or More! U.S. Orders Only * Offer Excludes Hazmat Shipments *

Menu

Vitamin E (VE) Colorimetric Assay Kit

SKU: E-BC-K033-M-96

To better serve you, we would like to discuss your specific requirement.
Please Contact Us for a quote.

Vitamin E (VE) Colorimetric Assay Kit

SKU # E-BC-K033-M
Detection Instrument Microplate reader (525-533 nm, optimum wavelength: 533 nm)
Detection Method Colorimetric method


Product Details

Properties

Synonyms VE
Sample Type Serum, plasma, tissue
Sensitivity 0.95 μg/mL
Detection Range 0.95-40 μg/mL
Detection Method Colorimetric method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 6.300% | Average intra-assay CV: 3.900%
Other instruments required Micropipettor, Centrifuge, Vortex mixer
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol, N-heptane
Storage 2-8℃
Valid period 12 months


Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.95-40 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only)

Sample type Dilution factor
Human serum 1
Mouse serum 1
Chicken serum 1
10% Mouse liver tissue homogenate 1
10% Mouse brain tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Rat lung tissue homogenate 1
10% Rat spleen tissue homogenate 1
10% Rat heart tissue homogenate 1

 

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

 

Detection Principle

Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. After colorimetric assay, VE content can be figured out according to the standard curve or calculated through formula.


Kit Components & Storage

Item Component Size 1(48 T) Size 2(96 T) Storage
Reagent 1 Chromogenic Agent Powder × 1 vial Powder × 1 vial 2-8℃, 12 months shading light
Reagent 2 Ferrum Reagent Powder × 1 vial Powder × 1 vial 2-8℃, 12 months shading light
Reagent 3 Stop Solution 1.5 mL × 1 vial 1.5 mL × 2 vials 2-8℃, 12 months
Reagent 4 Homogenized Medium 50 mL × 1 vial 50 mL × 2 vials 2-8℃, 12 months
Reagent 5 1 mg/mL VE Standard 0.4 mL × 1 vial 0.4 mL × 1 vial 2-8℃, 12 months shading light
Microplate 96 wells No requirement
Plate Sealer 2 pieces

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

 

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).

Parameters Sample 1 Sample 2 Sample 3
Mean (μg/mL) 2.60 24.50 32.50
%CV 4.3 3.7 3.7


Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Parameters Sample 1 Sample 2 Sample 3
Mean (μg/mL) 2.60 24.50 32.50
%CV 6.1 6.4 6.4


Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 97%.


Standard 1 Standard 2 Standard 3
Expected Conc. (μg/mL) 7.5 18 27.5
Observed Conc. (μg/mL) 7.1 17.8 26.7
Recovery rate (%) 95 99 97


Sensitivity

The analytical sensitivity of the assay is 0.95 μg/mL VE. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

 

Standard Curve:

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

Concentration (μg/mL) 0 5 10 15 20 25 30 40
Average OD 0.072 0.142 0.190 0.247 0.309 0.362 0.438 0.541
Absoluted OD 0 0.070 0.118 0.175 0.237 0.290 0.366 0.469