γ-Glutamyl Transferase (GGT/γ-GT) Activity Assay Kit

SKU #	E-BC-K126-M
Detection Instrument	Microplate reader (405 nm)
Detection Method	Colorimetric method

Product Details

Properties

Synonyms	GGT/γ-GT
Sample Type	Serum, plasma, animal tissue
Sensitivity	0.88 U/L
Detection Range	0.88-399.4 U/L
Detection Method	Colorimetric method
Assay type	Enzyme Activity
Assay time	20 min
Precision	Average inter-assay CV: 6.200% \| Average intra-assay CV: 4.200%
Other instruments required	Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator
Storage	2-8℃
Valid period	12 months

Images

GX Xu et al investigate the effect of endothelin receptor B in enhancing liver injury and pro-inflammatory. Activity of γ-glutamyl transferase of human serum was determined using γ-GT activity assay kit (E-BC-K126-M).

The activity of γ-GT was significantly increased in PBC patients. (**P<0.05)

Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.88-399.4 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Mouse serum	1
Human serum	1
Rat serum	1
Dog serum	1
Human plasma	1
Horse serum	1
Porcine serum	1
Human hydrothorax	1
10% Mouse liver tissue homogenate	1
10% Mouse heart tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Rat lung tissue homogenate	1

Note: The diluent is reagent 3.

Detection Principle

γ-GT catalyzes the transfer of gamma glutamyl group from glutamyl p-nitroaniline to N-glycyl glycine to produce p-nitroaniline, which has characteristic absorption peak at 405nm. The activity of γ-GT can be calculated according to the changing rate of absorbance value.

Kit Components & Storage

Item	Component	Size 1(48 T)	Size 2(96 T)	Storage
Reagent 1	Buffer Solution	15 mL ×1 vial	30 mL ×1 vial	2-8°C, 12 months
Reagent 2	Substrate	Powder ×1 vial	Powder ×2 vials	2-8°C, 12 months, shading light
Reagent 3	Extracting Solution	50 mL ×1 vial	50 mL ×2 vials	2-8°C, 12 months
Reagent 4	1.0 mmol/L p-Nitroaniline Standard Solution	1.5 mL ×1 vial	1.5 mL ×1 vial	2-8°C, 12 months
Reagent 5	Standard Diluent	10 mL ×1 vial	10 mL ×1 vial	2-8°C, 12 months
	Microplate	96 wells		No requirement
	Plate Sealer	2 pieces

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)

Parameters	Sample 1	Sample 2	Sample 3
Mean (U/L)	2.50	94.60	207.00
%CV	4.5	4.0	4.1

Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Parameters	Sample 1	Sample 2	Sample 3
Mean (U/L)	2.50	94.60	207.00
%CV	6.0	6.3	6.3

Sensitivity

The analytical sensitivity of the assay is 0.88 U/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

Standard Curve:

As the OD value of the standard curve may vary according to the conditions of the
actual assay performance (e.g. operator, pipetting technique or temperature effects),
so the standard curve and data are provided as below for reference only:

Concentration (μmol/L)	0	200	400	500	600	800	900	1000
OD value	0.042	0.364	0.656	0.844	1.051	1.355	1.485	1.678
OD value	0.042	0.367	0.710	0.862	1.027	1.351	1.474	1.606
Average OD	0.042	0.366	0.683	0.853	1.039	1.353	1.480	1.642
Absoluted OD	0.000	0.324	0.641	0.811	0.997	1.311	1.438	1.600

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