Anti-His(HHHHHH) FAST IP kit

Anti-His(HHHHHH) FAST IP kit

Background

Anti-His tag (HHHHHH) FAST IP kit consists of four components: Anti-His Affinity Agarose, Mouse IgG Affinity Agarose, His-Tag Rabbit pAb and HRP-labeled goat anti-rabbit secondary antibody. For rapid, efficient and specific immuno(co)precipitation of His-tagged fusion proteins.

Anti-His Affinity Agarose is made by covalently conjugating high-quality His-Tag Mouse mAb to agarose gel. It has the characteristics of high loading capacity, high specificity and stable properties; Mouse IgG Affinity Agarose is used as CoIP experimental control with stable properties; His-Tag Rabbit pAb antibody has the advantages of high specificity, high affinity, and high titer. The HRP-labeled goat anti-rabbit secondary antibody was purified through cross adsorption and only recognizes His Tag Rabbit pAb, without cross reactivity with the heavy and light chains of mouse monoclonal antibodies. The four components have undergone strict quality inspection and can be used individually; the set of the four components has the advantages of speed, simplicity and no interference with the strips.

Performance

1.         Scope of application

Immuno(co)precipitation of His-tagged fusion proteins.

The His tag can be located at the N-terminus, C-terminus or in the middle of the protein, such as N-terminal His fusion protein (His–Protein), C-terminal His fusion protein (Protein-His) and Met-modified N-terminal His fusion protein (Met-His– Protein). 

Suitable for secreted proteins.

2.       Antibody properties

His-Tag Mouse mAb: mouse IgG2a subtype;

His-Tag Rabbit pAb: rabbit IgG.

3.       Gel properties

Agarose gel particles, average particle size 100μm

4.       Loading Capacity

0.5mL Sepharose 4B agarose particles, covalently conjugated to 4mg Anti-His mouse monoclonal antibody. Can purify or precipitate at least 0.6mg His fusion protein.

Product Components

Note: Buffer is PBS containing 50% glycerol.

Storage

-20°C, 12 months.