Anti-V5(GKPIPNPLLGLDST) FAST IP kit(Beads)

Anti-V5(GKPIPNPLLGLDST)  FAST IP kit(Beads)

Background

Anti-V5 tag (GKPIPNPLLGLDST) FAST IP kit consists of four components: Anti-V5 immunomagnetic beads, Rabbit IgG magnetic beads, V5-Tag Mouse mAb and HRP-labeled goat anti-mouse secondary antibody for rapid, efficient and specific immuno(co)precipitation of V5 tag fusion proteins.

Anti-V5 immunomagnetic beads is made by covalently conjugating high-quality V5-Tag Rabbit pAb to immunomagnetic beads. It has the characteristics of high loading capacity, high specificity and stable properties; Rabbit IgG immunomagnetic beads are used as CoIP experimental control with stable properties; V5-Tag Mouse mAb antibody has the advantages of high specificity, high affinity, and high titer; HRP-labeled goat anti-mouse secondary antibody has been cross-adsorbed and purified, and only recognizes V5-Tag Mouse mAb, without cross reactivity with the heavy and light chains of rabbit polyclonal antibody. The four components have undergone strict quality inspection and can be used individually; the set of the four components has the advantages of fast, easyand no interference with the strips.

Performance

1.         Scope of application

Immuno(co)precipitation of V5-tagged fusion proteins.

The V5 tag can be located at the N-terminus, C-terminus or in the middle of the protein, such as N-terminal V5 fusion protein (V5–Protein), C-terminal V5 fusion protein (Protein-V5) and Met-modified N-terminal V5 fusion protein(Met-V5–Protein). 

Suitable for secreted proteins.

2.       Antibody properties

V5-Tag Rabbit pAb: rabbit IgG; V5-Tag Mouse mAb: mouse IgG2a subtype.

3.       Magnetic Beads properties

Magnetic beads, average particle size 3μm.

4.       Loading Capacity

1mL magnetic beads, covalently coupled to 4mg Anti-V5 rabbit polyclonal antibody. Precipitates at least 0.6mg V5 fusion protein

Product Components

Note: Buffer is PBS containing 50% glycerol.

Storage

4°C for 12 months