Bioneer AccuPower RT PreMix (480 T, 20 μl)

Bioneer AccuPower® RT PreMix (480 T, 20 μl)

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AccuPower^® RT PreMix contains premixed dried components essential for cDNA synthesis such as reverse transcriptase and RNase inhibitor, allowing RT reaction to simply start by just adding the RNA, primers, and D.W. Furthermore, by using the RNase H⁺ of M-MLV Reverse Transcriptase, template RNA is removed after the cDNA synthesis to minimize the effect of residual template RNA in PCR reaction.

※ This product is shipped at room temperature. The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.

※ A separate purchase of RT Primer is needed, since this product does not contain RT primer (Oligo dT, Random primer).

Features and Benefits

Easy-of-use

Amounts of reactants required for one PCR cycle included in each of the PCR tubes, allowing anyone to perform PCR tests easily simply by adding template DNA and primers.

Stability

Addition of stabilizer and RNase inhibitor for high resistance to degradation.

Reproducibility

Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables.

Applications

• First-strand synthesis of cDNA from RNA molecules (RT)
• Random priming reaction
• cDNA library construction
• Probe labeling
• mRNA 5’-end mapping by primer extension analysis
• PCR
• Real-time qPCR

Specifications