Bioneer AccuPrep Universal RNA Extraction Kit (100 reactions)
SKU: K-3140
Bioneer AccuPrep® Universal RNA Extraction Kit (100 reactions)
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AccuPrep® Universal RNA Extraction Kit allows rapid extraction of RNA from cultured cells, mammalian tissues, and plant tissues. The isolated RNA can be used for various experiments such as RT-PCR, quantitative real time RT-PCR, microarray analysis, etc. Spin column type
Features and Benefits
Flexible Sample Choice
Isolation of RNA from a wide variety of samples such as cultured cells, animal tissues, plant tissues, etc.
High Binding Capacity
Up to 120 µg using silica based RNA binding columns
Minimized Contamination
Columns Individually sterilized and packaged
Convenience
Ethanol already added to the Wash Buffer
Applications
- cDNA synthesis
- RT-PCR
- Quantative real-time RT-PCR
- Northern blot analysis
Specifications
Column loading volume | 800 µl |
---|---|
Max binding capacity | ~ 120 µg |
Elution volume | 30 µl - 100 µl |
Preparation time | ~ 20 min |
Sample | Amount | Typical yield | Purity |
---|---|---|---|
Cultured cells | 104 – 108 cells | 15 – 20 μg | A260/280 > 2.0 A260/230 > 1.7 |
Mammalian tissue | 25 – 50 mg | 10 - 60 μg | |
Plant tissue | 100 mg | 30 - 60 μg |
* Differences in the quantified value may occur depending on the types of samples.
Components
Components | K-3141 (50 reactions) | K-3140 (100 reactions) |
---|---|---|
RB Buffer (RNA Binding) | 30 ml x 1 ea | 60 ml x 1 ea |
RWA1 Buffer (1st Washing) | 40 ml x 1 ea | 80 ml x 1 ea |
RWA2 Buffer (2nd Washing) | 70 ml x 1 ea | 70 ml x 2 ea |
ER Buffer (Elution) | 10 ml x 1 ea | 20 ml x 1 ea |
AccuPrep® Binding Column-III | 50 ea | 100 ea |
1.5 ml Tube (for collection) | 50 ea | 100 ea |
User's Guide | 1 | 1 |