Bioneer CycleScript Reverse Transcriptase (50,000 Units)

Bioneer CycleScript™ Reverse Transcriptase (50,000 Units)

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CycleScript^™ Reverse Transcriptase uses a stabilizer to synthesize cDNA even at high temperature (55°C). Not only this product is capable of performing traditional fixed temperature reverse transcription (FTRT), but also cyclic reverse transcription(CRT) through sequential temperature change in 2-3 steps.

※This product is shipped in dry ice.

Features and Benefits

Flexible Reaction Conditions

Cyclic temperature reverse transcription reaction performs primer annealing at low temperature (15 to 40°C). As the secondary structure of template RNA is released through repeated application of high temperature (50 to 55°C), cDNA can be synthesized with more efficiency than the conventional 42°C reverse reaction. Furthermore, reverse transcription is also possible at a fixed temperature (22 ~ 55°C).

Stability

Higher thermostability than conventional reverse transcriptase capable of undergoing the reverse transcription up to 55 ℃.

Easy of Use

Shortened reaction time not requiring pre-incubation process of primer and RNA template during the performance of cyclic reverse transcription, with simplified procedure and reduced reaction time.

Reproducibility

Reproducible results with uniform quality products for each batch under the ISO 9001 quality system.

Applications

• First-strand synthesis of cDNA from RNA molecules
• RT-PCR
• Random priming reaction
• Library construction
• Probe labeling
• mRNA 5' end mapping by primer extension analysis.

Specifications

Components

5x Reaction Buffer: Tris-HCl, KCl, MgCl₂ (pH8.1)

100 mM DTT

10 mM dNTPs mix: 2.5 mM of each dNTP

Concentration

10,000 Unit (200 U/μl)

Storage Conditions

50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50 mM (NH₄)2_SO₄, Stabilizers.

Storage Temperature

-20°C

Unit Definition