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Bioneer ExiProgen EC-Disulfide Protein Synthesis Kit (8 reactions)
SKU: K-7330
Bioneer ExiProgen™ EC-Disulfide Protein Synthesis Kit (8 reactions)
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ExiProgen™ EC-Disulfide Protein Synthesis Kit allows automatic synthesis of proteins with disulfide bonds capable of acquiring maximum of 8 types in a single reaction. As proteins with 9 disulfide bonds can be easily synthesized, it is much more efficient to use then protein expression using cells. After mounting the kit including the template DNA to the ExiProgen™, by operating the Protocol No. 905, all the reactions from the expression, purification, and dialysis are fully automated, with the final target proteins recovered dissolved in the storage buffer.
※ This product is shipped in dry ice and ice pack by cartridge.
Features and Benefits
Disulfide bond protein synthesis
Regulation of the protein expression environment to form disulfide bonds
Fully automated system
Automatic synthesis & purification of the target protein only within 36 hours just by activating ExiProgen™ mounted with the cartridge having the template DNA.
Concurrent multiprotein synthesis/purification/dialysis
Simultaneous synthesis, purification, and dialysis of 1 ~ 8 different kinds of proteins with disulfide bonds
Reproducibility
Reproducible results through optimized protocols of automated devices to easily get about 50 μg protein.
Applications
- Antibody screening.
Specifications
Components
| Components | Storage Temperature | ||
| Kit 1 | Cartridge ① | 96 well x 1ea | 4℃ Storage |
| Dialysis tube | 1 pack (16 ea/pack) | ||
| Disposable filter tip | 1 pack (8 ea/pack) | Storage at room temperature | |
| Protection cover | 1 ea | ||
| Kit 2 | Cartridge ② | 96 well x 1ea | -20℃ (E. coli extract recommended at -70℃ or less) |
| *E. coli extract | 8-strip tube (Yellow) x 1 ea | ||
| Master mixture | 8-strip tube (Violet) x 1 ea | ||
| DEPC D.W. | 8-strip tube (White) x 1 ea | ||
| Storage buffer | 35 ml x 2 bottle | ||
| Positive control DNA | 1.5 ml tube x 1 ea | ||
*E. coli extract (8 strip tube): As T7 RNA polymerase and ribosome are included, it is recommended to be stored at temperatures below -70 ℃ immediately upon the receive. Performance will decrease upon repeated freezing and thawing.