Bioneer M-MLV Reverse Transcriptase (10,000 Units)
SKU: E-3121
Bioneer M-MLV Reverse Transcriptase (10,000 Units)
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Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase capable of using an RNA molecule as a template to synthesize a double-stranded DNA. It is ideal for use of first-strand synthesis cDNA for Reverse Transcriptase PCR and qRT-PCR
※This product is shipped in dry ice.
Features and Benefits
Optimized Buffer Delivery
Guaranteed reliable response by providing optimized for M-MLV reverse transcriptase
Reproducibility
Reproducible results with uniform quality products for each batch under the ISO 9001 quality system
Applications
- First strand cDNA synthesis from RNA, RT-PCR, and qRT-PCR.
Specifications
5' to 3' exonuclease activity | No |
3' to 5' exonuclease activity | No |
3' – A overhang | No |
Strand displacement | Yes |
Fragment size | Up to 9 kb |
Components
5X reaction buffer: Tris-HCl, KCl, MgCl2(pH 8.1) 100 mM DTT
10 mM dNTPs mix: 2.5 mM of each dNTP
Concentration
10,000 Units (200 U/μl)
Storage Conditions
50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50 mM (NH4)2SO4, Stabilizers.
Storage Temperature
-20°C