Bioneer RocketScript Reverse Transcriptase (10,000 Units)

Bioneer RocketScript™ Reverse Transcriptase (10,000 Units)

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RocketScript^TM Reverse Transcriptase is an independently developed thermostable reverse transcriptase derived from M-MLV. Its resistance to heat (42 ~ 70℃) allows effective synthesis of complex RNA structures with full-length cDNA.

※This product is shipped in dry ice.

Features and Benefits

Thermostability

Performance at comprehensive temperature conditions from 42 to 70 ℃ for cDNA synthesis with Rocketscript ™ series overcoming the limitation of traditional 42°C RT reactions.

User-friendly

Simplified procedure by including all components necessary for cDNA synthesis such as Thermostable Reverse Transcriptase, RNase inhibitor, etc.

Reproducibility

Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system.

Applications

• Gene synthesis
• First-strand synthesis of cDNA from RNA molecules (reverse transcription)
• RT-PCR
• Random priming reactions
• Library construction
• Probe labeling
• mRNA 5'-end mapping by primer extension analysis
• Real time PCR.

Specifications

Components

 5x Reaction Buffer: Tris-HCl, KCl, MgCl₂, Stabilizers (pH8.5)

100 mM DTT

10 mM dNTPs mix: 2.5 mM of each dNTP

Concentration

10,000 Units (200 U/μl)

Storage Conditions

50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, Stabilizers.

Storage Temperature

-20°C