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Calcium (Ca) Colorimetric Assay Kit– MSE Supplies LLC

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Calcium (Ca) Colorimetric Assay Kit

SKU: E-BC-K103-M-500

  • £26000
  • Save £4600


Calcium (Ca) Colorimetric Assay Kit

SKU # E-BC-K103-M
Detection Instrument Microplate reader (600-620 nm, optimum wavelength: 610 nm)
Detection Method Colorimetric method


Product Details

Properties

Synonyms Ca
Sample Type Serum, plasma, urine, tissue
Sensitivity 0.07 mmol/L
Detection Range 1.5-150 mmol/L
Detection Method Colorimetric method
Assay type Quantitative
Assay time 20 min
Precision Average inter-assay CV: 8.500% | Average intra-assay CV: 4.700%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Storage 2-8℃
Valid period 12 months


Images

TH Huang et al developed a surface decorated peptide nanoparticle that can be used for osteoporosis and diabetes therapy. Calcium (Ca) level in rat plasma was determined using Ca colorimetric assay kit (E-BC-K103-M).

The levels of Ca concentration was significantly reduced in PD5 group. (**P<0.01)

 

Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.07-1.2 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Dog serum 2-3
Human serum 3-6
Mouse serum 3-6
Human urine 4-8
20% Animal tissue homogenate 1

 

Note: The diluent is deionized water.

 

Detection Principle

Calcium ion in the sample bind to Methyl Thymol Blue (MTB) in alkaline solution and form blue complex. The blue complex has a specific absorption peak at 715nm and calcium content can be calculated by measuring the OD value at 610 nm.

 

Kit Components & Storage

Item Component Size 1(48 T) Size 2(96 T) Storage
Reagent 1 MTB Reagent 5 mL ×1 vial 10 mL ×1 vial 2-8°C, 12 months, shading light
Reagent 2 Alkali Reagent 10 mL ×1 vial 20 mL × 1 vial 2-8°C, 12 months
Reagent 3 Clarificant 1 mL ×1 vial 1 mL ×1 vial 2-8°C, 12 months
Reagent 4 2.5 mmol/L Calcium Standard 5 mL ×1 vial 10 mL ×1 vial 2-8°C, 12 months
Microplate 96 wells No requirement
Plate Sealer 2 pieces

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

 

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)

Parameters Sample 1 Sample 2 Sample 3
Mean (mmol/L) 0.35 0.86 1.05
%CV 4.8 4.7 4.6


Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Parameters Sample 1 Sample 2 Sample 3
Mean (mmol/L) 0.35 0.86 1.05
%CV 8.4 8.6 8.5

 

Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 99%


Standard 1 Standard 2 Standard 3
Expected Conc. (mmol/L) 0.25 0.5 0.95
Observed Conc. (mmol/L) 0.3 0.5 0.9
Recovery rate (%) 101 99 97


Sensitivity

The analytical sensitivity of the assay is 0.07 mmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

 

Standard Curve

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

Concentration (mmol/L) 0 0.2 0.3 0.4 0.6 0.8 1.0 1.2
OD value 0.225 0.246 0.265 0.273 0.297 0.321 0.348 0.365
0.227 0.242 0.255 0.275 0.297 0.323 0.344 0.369
Average OD 0.226 0.244 0.260 0.274 0.297 0.322 0.346 0.367
Absoluted OD 0 0.018 0.034 0.047 0.071 0.096 0.120 0.141