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Chromogenic Biotin Labeling Kit (50 KD Filtration Tube)– MSE Supplies LLC

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Chromogenic Biotin Labeling Kit (50 KD Filtration tube) - MSE Supplies LLC

Chromogenic Biotin Labeling Kit (50 KD Filtration Tube)

SKU: E-LK-B009C-10

  • £75000
  • Save £13200

Chromogenic Biotin Labeling Kit (50 KD Filtration Tube)

 

Introduction

Elabscience® Chromogenic Biotin Labeling Kit provides all the reagents required for labeling, which can label proteins containing primary amino-group (-NH2) molecules simply and effectively.

 

Technical Information

Technical Information Elabscience® Chromogenic Biotin Labeling Kit provides all the reagents required for labeling, which can label antibodies and other proteins containing primary amino-group (-NH2) molecules simply and effectively.
Applications Protein labeling/Antibody labeling (50 KD Filtration tube)
Principle
Labeling group Chromogenic Biotin
Spectrum

Characteristic

  • Fast: The whole process takes only 90 min.
  • Convenient: The dye has been activated and can be used directly. Filtration tube desalts without dialysis.
  • Flexible use: It can be used for both micro-labeling and large-scale labeling, and can label 0.1-1 mg proteins each time.
  • Biotin quantitative labeling: biotin with optimized structure and
    chromophore group can be quantified after labeling.

Materials Not Supplied

  1. Pipettor and tips (0.5-10μL, 2-20μL, 20-200μL, 200-1000μL).
  2. Ultraviolet spectrophotometer or nanodrop or multifunctional microplate reader.
  3. 37°C incubator.
  4. Centrifuge (centrifugal force up to 12,000×g).

 

 

Shipping & Storage Information

Storage 2~8°C/-20°C, shading light
Shipping Ice bag
Expiration date 12 months


Notes
  1. Please select the appropriate kit according to the molecular weight of the protein to be labeled. The kit provides a 50 KD Filtration tube.
  2. Chromogenic Biotin is susceptible to moisture hydrolysis failure, and should be stored at -20°C or -80°C with the desiccant. In order to prevent water vapor from condensing into the dye, it is necessary to equilibrate the dye to room before the experiment.
  3. The kit can also be used to label other proteins containing free amino groups. The specific labeling ratio is determined according to the number of available amino groups in the marker or set different molar ratios for labeling.
  4. The optimal molecular ratio of the dye and protein (150KD) recommended for labeling with this kit is 8:1, which can ensure that the dye can label the protein, and DOS is in the normal range, but it cannot ensure the best experimental results. The optimal labeling ratio may vary according to the difference of protein, and the user can optimize according to the actual situation.