Glucose Uptake Fluorometric Assay Kit
SKU: E-BC-F041-500
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Glucose Uptake Fluorometric Assay Kit
SKU # | E-BC-F041 |
Detection Instrument | Fluorescence microplate reader (Ex/Em=530 nm/590 nm) |
Detection Method | Fluorescence method |
Product Details
Properties
Sample Type | Cells |
Sensitivity | 0.02 nmol/μL |
Detection Range | 0.02-0.3 nmol/μL |
Detection Method | Fluorescence method |
Assay Time | 100 min |
Other Reagents Required | KRPH solution (20 mM Hepes, 5 mM KH2PO4, 1 mM MgSO4, 1 mM CaCl2, 136 mM NaCl, 4.7 mM KCl, 2% BSA, pH 7.4) |
Storage | This product can be stored at -20°C for 12 months with shading light. |
Valid period | 12 months |
Detection Principle
2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm.
Kit Components & Storage
Item | Component | Size 1 (96 T) | Size 2 (500 Assays) | Storage |
Reagent 1 | Acid Reagent | 10 mL ×1 vial | 55 mL ×1 vial | -20℃, 12 months |
Reagent 2 | Alkali Reagent | 10 mL × 1 vial | 55 mL × 1 vial | -20℃, 12 months |
Reagent 3 | Chromogenic Agent | 25 mL × 1 vial | 45 mL × 3 vials | -20℃, 12 months shading light |
Reagent 4 | Enzyme Reagent | Powder × 2 vials | Powder × 10 vials | -20℃, 12 months |
Reagent 5 | 10 mmol/L 2-DG | 1.5 mL × 1 vial | 8 mL × 1 vial | -20℃, 12 months |
Reagent 6 | 0.3 nmol/μL Standard | 2 mL × 1 vial | 5 mL × 2 vials | -20℃, 12 months |
Reagent 7 | KRPH Buffer Solution | 55 mL × 1 vial | 60 mL × 5 vials | -20℃, 12 months |
Black Microplate | 96 wells | No requirement | ||
Plate Sealer | 2 pieces |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Standard Curve:
As the fluorescence value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:
Concentration (nmol/L) | 0 | 0.06 | 0.12 | 0.15 | 0.18 | 0.21 | 0.24 | 0.3 |
Fluorescence value | 5176 | 8677 | 11778 | 13332 | 14730 | 16049 | 17242 | 19704 |
5211 | 8643 | 11635 | 13276 | 14717 | 15968 | 17037 | 19603 | |
Average fluorescence value | 5194 | 8660 | 11707 | 13304 | 14724 | 16009 | 17140 | 19654 |
Absoluted fluorescence value | 0 | 3467 | 6513 | 8111 | 9530 | 10815 | 11946 | 14460 |