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High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)
SKU: E-BC-K222-S-100
High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)
| SKU # | E-BC-K222-S |
| Detection Instrument | Spectrophotometer (600 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Synonyms | HDL-C |
| Sample Type | Serum, plasma, cells, culture supernatant, tissue |
| Detection Range | 0.065-3.8 mmol/L |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Storage | 2-8℃ |
| Valid period | 12 months |
Images
T Albrahim et al investigate the function of lycopene in modulating oxidative stress and inflammation in hypercholesterolemic rats. High density lipoprotein cholesterol (HDL-C) levels in rat serum was determined using HDL-C colorimetric assay kit (E-BC-K222-S).
| Experimental Groups | Control | LYC | HCD | HCD-LYC | HCD-AV |
| Total cholesterol (mg/dL) | 86.58 ± 9.52 | 88.52 ± 11.79 | 253.69 ± 26.67 | 135.46 ± 19.15 | 115.23 ± 20.74 |
| Triglyceride (mg/dL) | 104.63 ± 15.61 | 106.01 ± 23.76 | 356.76 ± 57.36 a | 186.38 ± 39.17 ab | 162.54 ± 12.17 ab |
| LDL-C (mg/dL) | 14.71 ± 12.44 | 16.90 ± 9.00 | 154.61 ± 25.14 a | 48.98 ± 22.41 ab | 40.98 ± 25.71 ab |
| vLDL-c (mg/dL) | 20.93 ± 3.12 | 21.20 ± 4.75 | 71.35 ± 11.47 a | 37.28 ± 7.83 ab | 32.51 ± 2.45 ab |
| HDL-c (mg/dL) | 50.95 ± 8.61 | 50.41 ± 7.07 | 27.73 ± 3.74 a | 49.20 ± 6.20 b | 41.74 ± 6,59 b |
| Athrogenic index | 0.75 ± 0.44 | 0.78 ± 0.29 | 8.24 ± 1.23 a | 1.77 ± 0.39 ab | 1.86 ± 0.83 ab |
| Glucose (mg/dL) | 96.07 ± 12.54 | 100.72 ± 10.11 | 167.76 ± 25.78 a | 114.66 ± 19.18 b | 150.18 ± 15.99 a |
| Insulin (ng/mL) | 5.62 ± 0.93 | 5.72 ± 0.98 | 7.79 ± 1.78 a | 5.96 ± 1.08 b | 6.64 ± 0.91 b |
| Urea (mg/dL) | 48.52 ± 8.90 | 43.75 ± 6.88 | 132.85 ± 14.48 | 76.50 ± 10.53 ab | 106.68 ± 17.62 ab |
| Creatinine (mg/dL) | 0.54 ± 0.04 | 0.52 ± 0.07 | 1.08 ± 0.19 a | 066. ± 0.07 b | 0.79 ± 0.07 ab |
HDL-C level was significantly decreased in HCD group. (P<0.05)
Dilution of Sample
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| Human serum | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Porcine serum | 1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Detection Principle
The generated red purple pigment have a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and the HDL-C content in the sample can be calculated.
Kit Components & Storage
| Item | Component | Specification | Storage |
| Reagent 1 | Enzyme Working Solution 1 | 75 mL × 1 vial | 2-8℃, 12 months shading light |
| Reagent 2 | Enzyme Working Solution 2 | 25 mL × 1 vial | 2-8℃, 12 months shading light |
| Reagent 3 | Cholesterol Standard (Refer to the label of reagent 3 for concentration) | Powder × 1 vial | 2-8℃, 12 months shading light |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.