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Human IgA(Immunoglobulin A) ELISA Kit
SKU: E-OSEL-H0009
Human IgA(Immunoglobulin A) ELISA Kit
| Detection Range | 0.78-50 ng/mL |
| Sensitivity | 0.14 ng/mL |
Product Details
Properties
| Assay type | Sandwich-ELISA |
| Format | 96T |
| Assay time | 1.5h |
| Detection range | 0.78-50 ng/mL |
| Sensitivity | 0.14 ng/mL |
| Sample type &Sample volume | serum, plasma, urine, saliva; 50μL |
| Specificity | This kit recognizes IgA in samples. No significant cross-reactivity or interference between IgA and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Application | This ELISA kit applies to the in vitro quantitative determination of IgA concentrations in serum, plasma, urine, saliva. |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgA. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IgA are added to the micro ELISA plate wells. Human IgA in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IgA. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human IgA in the samples is then determined by comparing the OD of the samples to the standard curve.
Kit Components and Storage
An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
| Reference Standard | 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
| Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
| HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
|
| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
| Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
|
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
Technical Data:
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IgA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IgA were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 2.35 | 4.49 | 21.37 | 2.45 | 4.10 | 20.27 |
| Standard deviation | 0.15 | 0.22 | 0.91 | 0.14 | 0.23 | 1.11 |
| CV (%) | 6.41 | 4.95 | 4.25 | 5.69 | 5.66 | 5.48 |
Recovery
The recovery of IgA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 97-110 | 102 |
| EDTA plasma(n=8) | 88-98 | 93 |
| Urine(n=8) | 89-104 | 96 |
Target Information
| Synonyms | Immunoglobulin A,IgA |
| Research Area | Cancer, Immunology |
Assay Procedures
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1. Add 50µL standard or sample to the wells, Immediately add 50µL of HRP linked Detection Ab working solution to each well. Incubate for 60 min at 37°C. |
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2. Aspirate and wash the plate for 5 times. |
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3. Add 90µL of Substrate Reagent. Incubate for about 15 min at 37°C. |
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4. Add 50µL Stop Solution. |
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5. Read the plate at 450nm immediately. Calculation of the results |