Total Price: $0.00
Contact Information
L-Lactic Acid (LA) Colorimetric Assay Kit (Whole Blood Samples)
SKU: E-BC-K043-S-100
To better serve you, we would like to discuss your specific requirement.
Please Contact Us for a quote.
L-Lactic Acid (LA) Colorimetric Assay Kit (Whole Blood Samples)
| SKU # | E-BC-K043-S |
| Detection Instrument | Spectrophotometer (530 nm) |
| Detection Method | Colorimetric method |
Product Details
Properties
| Sample Type | Whole blood |
| Sensitivity | 0.14 mmol/L |
| Detection Range | 0.14-7.0 mmol/L |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 60 min |
| Precision | Average inter-assay CV: 2% | Average intra-assay CV: 1.600% |
| Other instruments required | Micropipettor, Vortex mixer, Incubator, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of Sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.14-7.0 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| Rabbit whole blood | 1-2 |
| Mouse whole blood | 1-2 |
| Rat whole blood | 1-2 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Detection Principle
Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.
Kit Components & Storage
| Item | Component | Size 1 (50 assays) | Size 2 (100 assays) | Storage |
| Reagent 1 | Protein Precipitator | Powder × 1 vial | Powder × 2 vials | 2-8℃, 12 months |
| Reagent 2 | Enzyme Diluent | 60 mL × 1 vial | 60 mL × 2 vials | 2-8℃, 12 months |
| Reagent 3 | Enzyme Stock Solution |
0.6 mL × 1 vial | 1.2 mL × 1 vial | 2-8℃, 12 months |
| Reagent 4 | Chromogenic Agent | 12 mL × 1 vial | 24 mL × 1 vial | 2-8℃, 12 months shading light |
| Reagent 5 | 3 mmol/L Standard Solution |
1 mL ×1 vial | 1 mL ×1 vial | 2-8℃, 12 months |
| Reagent 6 | Stop Solution | 30 mL × 1 vial | 60 mL × 1 vial | 2-8℃, 12 months |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three rabbit whole blood samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 1.30 | 3.40 | 5.70 |
| %CV | 1.8 | 1.5 | 1.5 |
Inter-assay Precision
Three rabbit whole blood samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 1.30 | 3.40 | 5.70 |
| %CV | 2.1 | 2.3 | 1.6 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 101%.
| Sample 1 | Sample 2 | Sample 3 | |
| Expected Conc. (mmol/L) | 2.6 | 4.8 | 6.1 |
| Observed Conc. (mmol/L) | 2.6 | 4.8 | 6.3 |
| Recovery rate (%) | 99 | 100 | 104 |
Sensitivity
The analytical sensitivity of the assay is 0.14 mmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.