Thank you!

Your quote has been successfully submitted!

For products requiring additional information, our team will contact you within 1 business day

Failed

There was an error submitting your quote. Please try again.

Lactate Dehydrogenase (LDH) Activity Assay Kit– MSE Supplies LLC

Free Shipping on MSE PRO Online Orders of $500 or More! U.S. Orders Only * Offer Excludes Hazmat Shipments *

Menu

Lactate Dehydrogenase (LDH) Activity Assay Kit

SKU: E-BC-K046-S-100

To better serve you, we would like to discuss your specific requirement.
Please Contact Us for a quote.

Lactate Dehydrogenase (LDH) Activity Assay Kit

SKU # E-BC-K046-S
Detection Instrument Spectrophotometer (450 nm)
Detection Method Colorimetric method


Product Details

Properties

Synonyms LDH
Sample Type Serum, plasma, tissue, cells
Sensitivity 4 U/L
Detection Range 4-400 U/L
Detection Method Colorimetric method
Assay type Enzyme Activity
Assay time 70 min
Precision Average inter-assay CV: 7.200% | Average intra-assay CV: 2.200%
Other instruments required Micropipettor, Vortex mixer, Water bath
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

 

Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (4-400 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 10-15
Human plasma 10-15
Porcine serum 20-30
Rat serum 20-30
Rat plasma 20-30
10% Rat liver tissue homogenate 800-1000
10% Mouse kidney tissue homogenate 500-800
10% Rat lung tissue homogenate 300-500

 

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


Detection Principle

Lactate dehydrogenase (LDH) is an oxidoreductase. LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. LDH is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein. LDH is released into the blood by cells after tissue damage or erythrocyte hemolysis. Extracellular LDH activity is used to detect cell damage or cell death.


Kit Components & Storage

Item Component Size (100 Assays) Storage
Reagent 1 Substrate Buffer 30 mL × 1 vial 2-8°C, 12 months
Reagent 2 Coenzyme I Powder × 1 vial 2-8°C, 12 months
Reagent 3 Chromogenic Agent 30 mL × 1 vial 2-8°C, 12 months, shading light
Reagent 4 Alkali Reagent 30 mL × 1 vial 2-8°C, 12 months
Reagent 5 2 µmol/mL Pyruvic Acid Standard 5 mL × 1 vial 2-8°C, 12 months

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

 

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).

Parameters Sample 1 Sample 2 Sample 3
Mean (U/L) 26.00 154.00 287.00
%CV 2.3 2.2 2.1


Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Parameters Sample 1 Sample 2 Sample 3
Mean (U/L) 26.00 154.00 287.00
%CV 6.8 7.3 7.5


Sensitivity

The analytical sensitivity of the assay is 4 U/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

 

Standard Curve:

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

Concentration (μmol/L) 0 0.05 0.1 0.2 0.4 0.6 0.8
Average OD 0.106 0.162 0.224 0.346 0.555 0.794 0.953
Absoluted OD 0.000 0.057 0.118 0.241 0.450 0.689 0.848