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Protein Carbonyl Colorimetric Assay Kit (Tissue and Serum Samples)
SKU: E-BC-K117-M-96
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Protein Carbonyl Colorimetric Assay Kit (Tissue and Serum Samples)
| SKU # | E-BC-K117-M |
| Detection Instrument | Microplate reader (370 nm) |
| Detection Method | Colorimetric method |
Product Details
Properties
| Sample Type | Serum, plasma, hydrothorax, cell culture supernatant, tissue |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 140 min |
| Precision | Average inter-assay CV: 8.500% | Average intra-assay CV: 5.200% |
| Other instruments required | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
| Other reagents required |
Absolute ethanol, Ethyl acetate |
| Storage | 2-8℃ |
| Valid period | 12 months |
Images
S G Darband et al found that quercetin attenuated oxidative DNA damage in rats with DMH induced colon carcinogenesis, changes of markers of protein oxidation can be obtained. Protein carbonyl level of rat colon tissue was determined using protein carbonyl colorimetric assay kit (E-BC-K117-M).
The level of carbonyl was significantly increased (P<0.05) in DMH treated groups (G3) comparing to control group (G1). Quercetin administration has inhibitory effects on protein carbonyl level in different phase (G4-G6).
Detection Principle
The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.
Kit Components & Storage
| Item | Component | Size 2(96 T) | Storage |
| Reagent 1 | Homogenate Medium | 50 mL × 2 vials | 2-8°C, 12 months |
| Reagent 2 | Sulfates | Powder ×2 vials | 2-8°C, 12 months, shading light |
| Reagent 3 | DNPH Solution | 20 mL ×1 vial | 2-8°C, 12 months, shading light |
| Reagent 4 | Acid Reagent | 20 mL ×1 vial | 2-8°C, 12 months |
| Reagent 5 | Protein Precipitator | 60 mL ×1 vial | 2-8°C, 12 months |
| Reagent 6 | Denaturant | 50 mL ×3 vials | 2-8°C, 12 months |
| Microplate | 96 wells | No requirement | |
| Plate Sealer | 2 pieces |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.