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Pyruvate Decarboxylase (PDC) Activity Assay Kit
SKU: E-BC-K500-M-96
Pyruvate Decarboxylase (PDC) Activity Assay Kit
| SKU # | E-BC-K500-M |
| Detection Instrument | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Synonyms |
PDC |
| Sample type | Serum, plasma, tissue and cell samples |
| Sensitivity | 0.67 U/L |
| Detection range | 0.67-27.73 U/L |
| Detection Method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 15 min |
| Precision | Average inter-assay CV: 3.100% | Average intra-assay CV: 2.800% |
| Other instruments required | Vortex mixer, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl) |
| Storage | -20℃ |
| Valid period | 12 months |
Detection Principle
Pyruvate decarboxylase (PDC) mainly exists in yeast and is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylate to acetaldehyde, which reacts under the action of ethanol dehydrogenase (ADH), and catalyzes NADH to convert into NAD+. NADH has a characteristic absorption peak at 340 nm. The PDC activity can be calculated by measuring the OD value at 340 nm.
Kit Components & Storage
| Item | Component | Size 1 (48 T) | Size 2 (96 T) | Storage |
| Reagent 1 | Buffer Solution | 10 mL × 1 vial | 20 mL × 1 vial | -20°C, 12 months shading light |
| Reagent 2 | Substrate A | Powder × 1 vial | Powder × 2 vials | -20°C, 12 months shading light |
| Reagent 3 | Enzyme Reagent | Powder × 1 vial | Powder × 2 vials | -20°C, 12 months shading light |
| Reagent 4 | Substrate B | Powder × 1 vial | Powder × 2 vials | -20°C, 12 months shading light |
| UV Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (U/L) | 2.70 | 13.50 | 22.60 |
| %CV | 3.2 | 2.7 | 2.5 |
Inter-assay Precision
Three human serum samples were assayed 17 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (U/L) | 2.70 | 13.50 | 22.60 |
| %CV | 2.8 | 3.1 | 3.4 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 105%.
| Sample 1 | Sample 2 | Sample 3 | |
| Expected Conc. (U/L) | 7.4 | 19.3 | 25.5 |
| Observed Conc. (U/L) | 7.8 | 20.7 | 26.0 |
| Recovery rate (%) | 106 | 107 | 102 |
Sensitivity
The analytical sensitivity of the assay is 0.67 U/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.