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Recombinant Danio rerio Beta-actin/ACTB protein (His Tag)
SKU: PDEO100003-100
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Recombinant Danio rerio Beta-actin/ACTB protein (His Tag)
| SKU # | PDEO100003 |
| Expression Host | E. coli |
Description
| Synonyms | A26C1A;A26C1B;ACTBB;ACTB;Actin beta;Actin cytoplasmic 1;Actin;cytoplasmic 1;N-terminally processed;Actx;b actin;Beta cytoskeletal actin;Beta-actin;BRWS1;E430023M04Rik;MGC128179;PS1TP5 binding protein 1;PS1TP5BP1 |
| Species | Danio rerio |
| Expression_host | E.coli |
| Sequence | Met1-Phe375 |
| Accession | Q7ZVF9 |
| Mol_Mass | 43.1 kDa |
| AP_Mol_Mass | 42-48 kDa |
| Tag | N-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA