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Recombinant Human ACP1/LMW-PTP Protein (GST Tag)(Active)
SKU: PKSH031337-100
Recombinant Human ACP1/LMW-PTP Protein (GST Tag)(Active)
| SKU # | PKSH031337 |
| Expression Host | E.coli |
Description
| Synonyms | ACP1, Adipocyte Acid Phosphatase, HAAP, LMW-PTP, LMW-PTPase, Low Molecular Weight Cytosolic Acid Phosphatase, Low Molecular Weight Phosphotyrosine Protein Phosphatase, Red Cell Acid Phosphatase 1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-His 158 |
| Accession | AAI06012.1 |
| Calculated Molecular Weight | 44.3 kDa |
| Observed Molecular Weight | 40 kDa |
| Tag | N-GST |
| Bio-activity | Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is > 65, 000 pmol/min/μg. |
Properties
| Purity | > 88 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 8.0 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
The low molecular weight phosphotyrosine phosphatase (LMW-PTP), also known as Acid phosphatase 1 (ACP1), belongs to the low molecular weight phosphotyrosine protein phosphatase family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. ACP1/LMW-PTP is an enzyme involved in platelet-derived growth factor-induced mitogenesis and cytoskeleton rearrangement. LMW-PTP is able to specifically bind and dephosphorylate activated PDGF receptor, thus modulating PDGF-induced mitogenesis. In vitro, LMW-PTP was found to efficiently dephosphorylate activated FcgammaRIIA and LAT, but not Syk or phospholipase Cgamma2. The overexpression of LMW-PTP inhibited activation of Syk downstream of FcgammaRIIA and reduced intracellular Ca(2+) mobilization. It been demonstrated that LMW-PTP is responsible for FcgammaRIIA dephosphorylation, and is implicated in the down-regulation of cell activation mediated by this ITAM-bearing immunoreceptor. In addition, ACP1 is a highly polymorphic phosphatase that is especially abundant in the central nervous system and is known to be involved in several signal transduction pathways.