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Recombinant Human ADA protein (His tag)
SKU: PDEH100156-100
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Recombinant Human ADA protein (His tag)
| SKU # | PDEH100156 |
| Expression Host | E. coli |
Description
| Synonyms | ADA;ADA1;adenine deaminase;Adenosine aminohydrolase;Adenosine Deaminase;EC 3.5.4.4 |
| Species | Human |
| Expression_host | E.coli |
| Sequence | Lys 11-Val 280 |
| Accession | P00813 |
| Mol_Mass | 29.6 kDa |
| AP_Mol_Mass | 32 kDa |
| Tag | N-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
Adenosine Deaminase (ADA, adenosine aminohydrolase) is one of the key enzymes of purine nucleotide catabolism. It catalyses the hydrolytic deamination of adenosine and deoxy?adenosine to inosine and deoxyinosine. ADA is expressed in virtually all tissues and is expressed at high levels in T-lymphocytes. Adenosine Deaminase deficiency can cause a form of SCID (severe combined immunodeficiency) and lymphopenia in both B- and T-cell lineages ADA can be used as a sensitive diagnostic marker for tuberculous pleuritis. Although it is primarily a cytosolic enzyme, ADA is known to be a positive regulator of T-cell co?activation due to its binding to CD26 at the cell surface. The interaction of ADA with CD26 regulates lymphocyte-epithelial cell adhesion.