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Recombinant Human APE1/APE Protein (His Tag)
SKU: PKSH030851-100
Recombinant Human APE1/APE Protein (His Tag)
| SKU # | PKSH030851 |
| Expression Host | E.coli |
Description
| Synonyms | AP Endonuclease 1, APE, APE-1REF-1, APE1, APEN, APEX, APEX Nuclease, APEX1, APX, Apurinic-Apyrimidinic Endonuclease 1, DNA-(Apurinic or Apyrimidinic Site) Lyase, HAP1, REF1, Redox Factor-1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Pro2-Leu 318 |
| Accession | P27695 |
| Calculated Molecular Weight | 37.0 kDa |
| Observed Molecular Weight | 37 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 92 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease; DNA 3' repair diesterase; 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously; the levels varied significantly; suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis; relatively high levels in the thymus; spleen; kidney and brain; and the lowest level in the liver in rats. However; the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain; especially in the hippocampal formation; to regulate some brain functions by redox-activation.