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Recombinant Human B7 Homolog 4/B7-H4/VTCN1 (C-Fc-Avi) Biotinylated
SKU: PKSH033983-100
Recombinant Human B7 Homolog 4/B7-H4/VTCN1 (C-Fc-Avi) Biotinylated
| SKU # | PKSH033983 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | B7-H4, B7H4T-cell costimulatory molecule B7x, B7S1, B7S1VCTN1, B7XPRO1291, B7h.5, B7x, FLJ22418, Immune costimulatory protein B7-H4, Protein B7S1, T cell costimulatory molecule B7x, V-set domain containing T cell activation inhibitor 1, V-set domain-containing , Vtcn1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Phe29-Ala258 |
| Accession | Q7Z7D3 |
| Calculated Molecular Weight | 54.1 kDa |
| Observed Molecular Weight | 70-95 kDa |
| Tag | C-Fc-Avi |
| Bio-activity | Immobilized Anti-Human B7-H4 mAb at 2μg/ml (100 μl/well) can bind Biotinylated Human B7-H4-Fc-Avi. The ED50 of Biotinylated Human B7-H4-Fc-Avi is 0.23 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
B7 Homolog 4 (B7-H4) is glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H4 consists of a 235 amino acid (aa) extracellular domain (ECD) with two Ig-like V-type domains, a 21 aa transmembrane segment, and a 2 aa cytoplasmic tail. It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.