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Recombinant Human BDNF Protein (with pro peptide)(Active)
SKU: PKSH033559-50
Recombinant Human BDNF Protein (with pro peptide)(Active)
| SKU # | PKSH033559 |
| Expression Host | E.coli |
Description
| Synonyms | Abrineurin, BDNF, Brain-Derived Neurotrophic Factor |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala19-Arg247(R125A,R127A,R128A) |
| Accession | P23560 |
| Calculated Molecular Weight | 25.6 kDa |
| Observed Molecular Weight | 28 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM PB, 10% Trehalose, 100mM NaCl, 0.02% Tween 80, pH 6.0. |
| Reconstitution | Not Applicable |
Background
The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis; whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival; growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects; Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.