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Recombinant Human Carboxypeptidase A1/CPA1 Protein (His Tag)(Active)
SKU: PKSH031565-50
Recombinant Human Carboxypeptidase A1/CPA1 Protein (His Tag)(Active)
| SKU # | PKSH031565 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CPA, CPA1, Carboxypeptidase A1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Tyr 419 |
| Accession | NP_001859.1 |
| Calculated Molecular Weight | 47.0 kDa |
| Observed Molecular Weight | 43 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5, 5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is > 3, 500 pmoles/min/μg. |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Carboxypeptidase A1 （CPA1）is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.