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Recombinant Human CCL8/MCP-2 Protein (Human Cells, His Tag)
SKU: PKSH032202-50
Recombinant Human CCL8/MCP-2 Protein (Human Cells, His Tag)
| SKU # | PKSH032202 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | C-C Motif Chemokine 8, CCL8, HC14, MCP-2, MCP2, Monocyte Chemoattractant Protein 2, Monocyte Chemotactic Protein 2, SCYA10, SCYA8, Small-Inducible Cytokine A8 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gln24-Pro99 |
| Accession | AAI26243.1 |
| Calculated Molecular Weight | 10.0 kDa |
| Observed Molecular Weight | 13 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM EDTA, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Human Chemokine (C-C Motif) Ligand 8 (CCL8) is produced by human MG63 osteosarcoma cells. CCL8 shares 62% and 58% amino acid sequence identity with MCP-1 and MCP-3; respectively. All three MCP proteins are monocyte chemoattractants. CCL8 is chemotactic for and activates many different immune cells; including mast cells; eosinophils and basophils; which are implicated in allergic response; and monocytes; T cells; and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different cell surface receptors including CCR1; CCR2B and CCR5.