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Recombinant Human CD4/LEU3 Protein (His Tag)
SKU: PKSH033506-50
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Recombinant Human CD4/LEU3 Protein (His Tag)
| SKU # | PKSH033506 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD4, CD4mut, Scd4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Lys26-Trp390 |
| Accession | P01730 |
| Calculated Molecular Weight | 41.7 kDa |
| Observed Molecular Weight | 58 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CD4 is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. Mature human CD4 consists of a 371 amino acid extracellular region containing four immunoglobulin-like domains; a 22 aa transmembrane segment; and a 40 aa cytoplasmic domain. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. CD4 binds directly to MHC class II molecules on antigen presenting cells (10). This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and; in human; as a co-receptor for the gp120 surface glycoprotein of HIV-1.