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Recombinant Human CDCP1/CD318 Protein (aa 1-343, His Tag)
SKU: PKSH030670-100
Recombinant Human CDCP1/CD318 Protein (aa 1-343, His Tag)
| SKU # | PKSH030670 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD318, CUB domain-containing protein 1, Membrane glycoprotein gp140, SIMA135, Subtractive immunization M plus HEp3-associated 135 kDa protein, TRASK, Transmembrane and associated with src kinases |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Glu343 |
| Accession | Q9H5V8-3 |
| Calculated Molecular Weight | 36.4 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CDCP1 contains three extracellular CUB domains. It is a putative stem cell marker that is highly expressed in some human cancer cells and in both; typical and atypical (cancerous) colons. It interacts with CDH2/N-cadherin; CDH3/P-cadherin; SDC1/syndecan-1; SDC4/syndecan-4 and the serine protease ST14/MT-SP1. It also interacts with SRC and PRKCG/protein kinase C gamma. CDCP1 is taken as a key regulator of EGF/EGFR-induced cell migration. It has been shown that signaling via EGF/EGFR induces migration of ovarian cancer Caov3 and OVCA420 cells with concomitant up-regulation of CDCP1 mRNA and protein. Consistent with a role in cell migration CDCP1 relocates from cell-cell junctions to punctate structures on filopodia after activation of EGFR. It may be involved in cell adhesion and cell matrix association. It also may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. It has been taken as a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets.