Recombinant Human CLEC10A/CD301 Protein (Fc Tag)
SKU: PKSH031411-50
Recombinant Human CLEC10A/CD301 Protein (Fc Tag)
SKU # | PKSH031411 |
Expression Host | HEK293 Cells |
Description
Synonyms | C-Type Lectin Domain Family 10 Member A, C-Type Lectin Superfamily Member 14, CD301, CLEC10A, CLECSF13, CLECSF14, HML, Macrophage Lectin 2 |
Species | Human |
Expression Host | HEK293 Cells |
Sequence | Gln61-His292 |
Accession | Q8IUN9-2 |
Calculated Molecular Weight | 54.6 kDa |
Observed Molecular Weight | 58 kDa |
Tag | N-hFc |
Bio-activity | Not validated for activity |
Properties
Purity | > 85 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background
CLEC10A, also known as the macrophage galactose-type calcium-type lectins (MGLs; CD301) constitute a unique class of C-type lectins because of their specificity for galactose and its structural homologues. MGLs/CD301 is a type II transmembrane glycoproteins and is expressed on macrophages and related cells of myeloid origins, particularly immature dendritic cells (DCs). There are 2 homologues: MGL1 and MGL2 (CD301a and CD301b) in mice. MGL1/CD301a induces both the production and secretion of interleukin (IL)-10. MGL1/CD301a plays a protective role against colitis by effectively inducing IL-10 production by colonic lamina propria macrophages in response to invading commensal bacteria.