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Recombinant Human CXADR/CAR Protein (His Tag)(Active)
SKU: PKSH031424-100
Recombinant Human CXADR/CAR Protein (His Tag)(Active)
| SKU # | PKSH031424 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CAR, CVB3-Binding Protein, CXADR, Coxsackievirus B-Adenovirus Receptor, Coxsackievirus and Adenovirus Receptor, HCVADR, hCAR |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Gly 237 |
| Accession | NP_001329.1 |
| Calculated Molecular Weight | 25.6 kDa |
| Observed Molecular Weight | 35 kDa |
| Tag | C-His |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 5 x 104 cells/well are added to CXADR coated plates (4 μg/ml and 100 μl/well), approximately 20%-40% will adhere specifically after 60 minutes at 37 ℃. |
Properties
| Purity | > 92 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 90% sequence identity with the corresponding regions of rat, human CXADR.