Recombinant Human Cytochrome b5 B/CYB5B Protein (His Tag)
SKU: PKSH032333-50
Recombinant Human Cytochrome b5 B/CYB5B Protein (His Tag)
SKU # | PKSH032333 |
Expression Host | HEK293 Cells |
Description
Synonyms | Cytochrome b5 outer mitochondrial membrane isoform, OMB5 |
Species | Human |
Expression Host | HEK293 Cells |
Sequence | Lys12-Cys118 |
Accession | AAH04373.1 |
Calculated Molecular Weight | 13.1 kDa |
Observed Molecular Weight | 22 kDa |
Tag | C-His |
Bio-activity | Not validated for activity |
Properties
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background
Cytochrome b5 type B (CYB5B) is a membrane of the cytochrome b5 family. It contains 1 cytochrome b5 heme-binding domain. Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. In the mitochondrion of eukaryotes and in aerobic prokaryotes, cytochrome b is a component of respiratory chain complex III also known as the bc1 complex or ubiquinol-cytochrome c reductase.