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Recombinant Human ERN1/IRE1 Protein (aa 465-977, His & GST Tag)(Active)
SKU: PKSH030346-50
Recombinant Human ERN1/IRE1 Protein (aa 465-977, His & GST Tag)(Active)
| SKU # | PKSH030346 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | IRE1, IRE1P, IRE1a, hIRE1p |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Pro 465-Leu 977 |
| Accession | O75460-1 |
| Calculated Molecular Weight | 86.0 kDa |
| Observed Molecular Weight | 90 kDa |
| Tag | N-His-GST |
| Bio-activity | The specific activity was determined to be 5 nmol/min/mg using MBP as substrate |
Properties
| Purity | > 80 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4 |
| Reconstitution | Not Applicable |
Background
The encoded protein contains two functional catalytic domains; a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins; regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.