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Recombinant Human ERN1/IRE1 Protein (aa 465-977)(Active)
SKU: PKSH030975-50
Recombinant Human ERN1/IRE1 Protein (aa 465-977)(Active)
| SKU # | PKSH030975 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | IRE1, IRE1P, IRE1a, hIRE1p |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Pro 465-Leu 977 |
| Accession | O75460-1 |
| Calculated Molecular Weight | 58.3 kDa |
| Observed Molecular Weight | 65 kDa |
| Tag | None |
| Bio-activity | 1. Kinase activity untested 2. Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate. |
Properties
| Purity | > 80 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Trypsin-3; also known as Trypsin III; brain trypsinogen; Serine protease 3 and PRSS3; is a secreted protein which belongs to thepeptidase S1 family. Trypsin-3 / PRSS3 is expressed is in pancreas and brain. It contains onepeptidase S1 domain. Trypsin-3 / PRSS3 can degrade intrapancreatic trypsin inhibitors that protect against CP. Genetic variants that cause higher mesotrypsin activity might increase the risk for chronic pancreatitis (CP). A sustained imbalance of pancreatic proteases and their inhibitors seems to be important for the development of CP. The trypsin inhibitor-degrading activity qualified PRSS3 as a candidate for a novel CP susceptibility gene. Trypsin-3 / PRSS3 has been implicated as a putative tumor suppressor gene due to its loss of expression; which is correlated with promoter hypermethylation; in esophageal squamous cell carcinoma and gastric adenocarcinoma.