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Recombinant Human FGF-4/FGF4 Protein (Active)
SKU: PKSH032442-50
Recombinant Human FGF-4/FGF4 Protein (Active)
| SKU # | PKSH032442 |
| Expression Host | E.coli |
Description
| Synonyms | FGF-4, FGF4, Fibroblast growth factor 4, HBGF-4, HST, HST-1, HSTF-1, HSTF1, Heparin secretory-transforming protein 1, Heparin-binding growth factor 4, KS3 , Transforming protein KS3 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ser54-Leu206 |
| Accession | P08620 |
| Calculated Molecular Weight | 16.9 kDa |
| Observed Molecular Weight | 16 kDa |
| Tag | None |
| Bio-activity | Measured in a cell proliferation assay using MCF7 Human breast cancer cells The ED50 for this effect is 2-20 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.01 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, 500mM NaCl, pH7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Fibroblast growth factor 4(FGF-4) is a heparin binding member of the FGF family. The human FGF4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus. Mature human FGF4 shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell selfrenewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease.