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Recombinant Human FGF-7/KGF Protein (His Tag)(Active)
SKU: PKSH032445-50
Recombinant Human FGF-7/KGF Protein (His Tag)(Active)
| SKU # | PKSH032445 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | FGF-7, FGF7, Fibroblast growth factor 7, HBGF-7, Heparin-binding growth factor 7, Keratinocyte growth factor |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Cys32-Thr194 |
| Accession | P21781 |
| Calculated Molecular Weight | 20.0 kDa |
| Observed Molecular Weight | 20-27 kDa |
| Tag | C-His |
| Bio-activity | Measured in a cell proliferation assay using HaCaT cells. The ED50 for this effect is 10.94 ng/ml. |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Fibroblast growth factor 7 (FGF7) is a secreted protein which is mainly located in epithelial cells and belongs to the heparin-binding growth factors family. FGF family members possess broad mitogenic and cell survival activities; and are involved in a variety of biological processes; including embryonic development; cell growth; morphogenesis; tissue repair; tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor; whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. It is possible major paracrine effector of normal epithelial cell proliferation.