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Recombinant Human Galectin-3/LGALS3 Protein, Low Endotoxin(Active)
SKU: PKSH031707-100
Recombinant Human Galectin-3/LGALS3 Protein, Low Endotoxin(Active)
| SKU # | PKSH031707 |
| Expression Host | E.coli |
Description
| Synonyms | 35 kDa Lectin, CBP35, Carbohydrate-Binding Protein 35, GAL3, GALBP, GALIG, Gal-3, Galactose-Specific Lectin 3, Galactoside-Binding Protein, Galectin-3, IgE-Binding Protein, L-31, L31, LGALS2, LGALS3, Laminin-Binding Protein, Lectin L-29, MAC2, Mac-2 Antigen, P35 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala 2-Ile 250 |
| Accession | P17931 |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 32 kDa |
| Tag | N-His |
| Bio-activity | Measured by its ability to chemoattract human PBMC using a concentration range of 2.5-25 μg/mL. Note: Results may vary from different PBMC donors. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Leukotriene A-4 hydrolase; also known as LTA-4 hydrolase; Leukotriene A (4) hydrolase; LTA4H and LTA4; is cytoplasm protein which belongs to thepeptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs; arachidonic acid was converted into the unstable intermediate epoxide LTA4; which may in turn be conjugated with glutathione to form the spasmogenic LTC4; or hydrolyzed into the proinflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium; and also modulates immune responses. As a bifunctional zinc metalloenzyme; LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.