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Recombinant Human GM-CSF/CSF2 Protein (His Tag, Cells)(Active)– MSE Supplies LLC

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Recombinant Human GM-CSF/CSF2 Protein (His Tag, Cells)(Active)

SKU: PKSH032502-50

  • £24800
  • Save £2700



Recombinant Human GM-CSF/CSF2 Protein (His Tag, Cells)(Active)

 

SKU # PKSH032502
Expression Host HEK293 Cells

 

 

Description

Synonyms CSF, CSF2, Colony-Stimulating Factor, GM-CSF, GMCSF, Granulocyte-Macrophage Colony-Stimulating Factor, Molgramostin, Sargramostim
Species Human
Expression Host HEK293 Cells
Sequence Ala18-Glu144
Accession P04141
Calculated Molecular Weight 15.5 kDa
Observed Molecular Weight 17-30 kDa
Tag C-His
Bio-activity Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 6-30 pg/ml.
  

 

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 0.01 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.



Background

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells; B cells; macrophages; mast cells; endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors; GM-CSF is also a growth factor for erythroid; megakaryocyte and eosinophil progenitors. On mature hematopoietic; monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally; GM-CSF can also stimulate the proliferation of a number of tumor cell lines; including osteogenic sarcoma; carcinoma and adenocarcinoma cell lines.