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Recombinant Human GPNMB Protein (aa 22-486, His Tag)
SKU: PKSH033371-50
Recombinant Human GPNMB Protein (aa 22-486, His Tag)
| SKU # | PKSH033371 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | GPNMB, HGFIN, NMB, Osteoactivin, Transmembrane Glycoprotein HGFIN, Transmembrane Glycoprotein NMB |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ala22-Pro486 |
| Accession | Q14956 |
| Calculated Molecular Weight | 53.1 kDa |
| Observed Molecular Weight | 80-120 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Osteoactivin is an intracellular glycoprotein belongs to the NMB/pMEL-17 family; which is asscociated with cell endosomal/lysomal compartments. Human Osteoactivin is a 560 amino acid type I transmembrane protein; and one alternate splice form shows a 12 amino acid insert between amino acid 339-340. An additional 206 amino acid isoform shows a mutation at position 181 that results in a 26 amino acid substitution for the C-terminal 380 amino acids. Cells knowns to express Osteoactivin include fibroblast; osteoblasts; myeloid dendritic cell; melanocytes; plus fetal chondrocytes and stratum basale keratinocytes; macrophages/keratinocytes.