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Recombinant Human GRK6/GPRK6 Protein (His & GST Tag)(Active)
SKU: PKSH030350-50
Recombinant Human GRK6/GPRK6 Protein (His & GST Tag)(Active)
| SKU # | PKSH030350 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | GPRK6 |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Met 1-Arg 589 |
| Accession | P43250-2 |
| Calculated Molecular Weight | 95.1 kDa |
| Observed Molecular Weight | 85 kDa |
| Tag | N-His-GST |
| Bio-activity | The specific activity was determined to be 7 nmol/min/mg using casein as substrate. |
Properties
| Purity | > 93 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, 2mM GSH, 0.5mM PMSF, pH 7.4 |
| Reconstitution | Not Applicable |
Background
G protein-coupled receptor kinase 6, also known as G protein-coupled receptor kinase GRK6, GRK6 and GPRK6, is a lipid-anchor protein which belongs to theprotein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRK6 / GPRK6 contains oneAGC-kinase C-terminal domain, oneprotein kinase domain and oneRGS domain. This protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described. GRK6 / GPRK6 is widely expressed. GRK6 / GPRK6 silencing causes suppression of signal transducer and activator of transcription 3 (STAT3) phosphorylation associated with reduction in MCL1 levels and phosphorylation, illustrating a potent mechanism for the cytotoxicity of GRK6 inhibition in multiple myeloma (MM) tumor cells. GRK6 also appears to be involved in responses to morphine. Inhibition of GRK6 represents a uniquely targeted novel therapeutic strategy in human multiple myeloma.