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Recombinant Human Hexokinase-3/HK3 Protein (His & GST Tag)
SKU: PKSH030376-50
Recombinant Human Hexokinase-3/HK3 Protein (His & GST Tag)
| SKU # | PKSH030376 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | HKIII, HXK3 |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Met 1-Val 923 |
| Accession | P52790 |
| Calculated Molecular Weight | 127 kDa |
| Observed Molecular Weight | 115 kDa |
| Tag | N-His-GST |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 7.4 |
| Reconstitution | Not Applicable |
Background
Hexokinase-3, also known as Hexokinase type III, HKIII and HK3, is a protein which belongs to thehexokinase family. Hexokinase-3 / HK3 is anenzymewhich in humans is encoded by theHK2gene. Hexokinases phosphorylate glucoseto produceglucose 6-phosphate, committing glucose to theglycolytic pathway. In mammalian tissues hexokinase exists as four isoenzymes encoded by distinct genes. These proteins are homologous and are organized in two homologous domains, with the exception of hexokinase type IV which has only one. This organization is believed to be the result of a duplication and tandem fusion event involving the gene encoding for the ancestral hexokinase. The gene encodes hexokinase-3. Similar to hexokinases-1 and hexokinases-2, thisallostericenzyme is inhibited by its product glucose 6-phosphate.