Recombinant Human HMGB1 Protein (MARI Tag)
SKU: PKSH032544-50
Recombinant Human HMGB1 Protein (MARI Tag)
SKU # | PKSH032544 |
Expression Host | E.coli |
Description
Synonyms | HMG-1, HMG1, HMGB1, High Mobility Group Protein 1, High Mobility Group Protein B1 |
Species | Human |
Expression Host | E.coli |
Sequence | Pro92-Val176 |
Accession | P09429 |
Calculated Molecular Weight | 10.0 kDa |
Observed Molecular Weight | 13 kDa |
Tag | N-MARI |
Bio-activity | Not validated for activity |
Properties
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from a 0.2 μm filtered solution of 50mM HEPES-Na, 500mM NaCl, 0.6mM DTT, pH 7.9. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background
High mobility group protein B1 is a member of the HMGB family consisting of three members; HMGB1; HMGB2 and HMGB3.It Contains 2 HMG box DNA-binding domains entitled box A and box B and It is a highly negative-charged C terminus. As a nuclear protein; HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile; it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte; T cell; dendritic cell activities in inflammatory responses.