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Recombinant Human ICOS Ligand/ICOSL Protein (His Tag)(Active)
SKU: PKSH031085-100
Recombinant Human ICOS Ligand/ICOSL Protein (His Tag)(Active)
| SKU # | PKSH031085 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | B7 Homolog 2, B7-H2, B7-Like Protein Gl50, B7-Related Protein 1, B7H2, B7RP-1, B7RP1, CD275, ICOS Ligand, ICOSL, ICOSLG, KIAA0653 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Ser 258 |
| Accession | NP_056074.1 |
| Calculated Molecular Weight | 28.0 kDa |
| Observed Molecular Weight | 50-60 kDa |
| Tag | C-His |
| Bio-activity | Immobilized human B7-H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 1. 6-200 ng/ml. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.