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Recombinant Human IDH1 Protein (Human Cells, His Tag)
SKU: PKSH033345-50
Recombinant Human IDH1 Protein (Human Cells, His Tag)
| SKU # | PKSH033345 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Cytosolic NADP-Isocitrate Dehydrogenase, IDH, IDH1, IDP, Isocitrate Dehydrogenase [NADP] Cytoplasmic, NADP(+)-Specific ICDH, Oxalosuccinate Decarboxylase, PICD |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Leu414 |
| Accession | O75874 |
| Calculated Molecular Weight | 47.7 kDa |
| Observed Molecular Weight | 45 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.05% Brij-35, 10% Glycerol, 1mM DTT, pH8.0. |
| Reconstitution | Not Applicable |
Background
Isocitrate Dehydrogenase [NADP] Cytoplasmic (IDH1) belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 exists as a homodimer; binding one magnesium or manganese ion per subunit. Mutations of IDH1 have been shown to cause metaphyseal chondromatosis with aciduria and are involved in the development of glioma IDH plays a role in the regeneration of NADPH for intraperoxisomal reductions; such as the conversion of 2; 4-dienoyl-CoAs to 3-enoyl-CoAs; as well as in peroxisomal reactions that consume 2-oxoglutarate; namely the α-hydroxylation of phytanic acid.