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Recombinant Human IDH1 Protein (R132H, C-8His)
SKU: PKSH032660-50
Recombinant Human IDH1 Protein (R132H, C-8His)
| SKU # | PKSH032660 |
| Expression Host | E.coli |
Description
| Synonyms | Cytosolic NADP-Isocitrate Dehydrogenase, IDH, IDH1, IDP, Isocitrate Dehydrogenase [NADP] Cytoplasmic, NADP(+)-Specific ICDH, Oxalosuccinate Decarboxylase, PICD |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met1-Leu414(Arg132His) |
| Accession | O75874 |
| Calculated Molecular Weight | 48.1 kDa |
| Observed Molecular Weight | 40-50 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 4% Sucrose, 50% glycerol, 0.02% Tween 80, pH 8.0. |
| Reconstitution | Not Applicable |
Background
Isocitrate Dehydrogenase [NADP] Cytoplasmic (IDH1) belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 exists as a homodimer; binding one magnesium or manganese ion per subunit. Mutations of IDH1 have been shown to cause metaphyseal chondromatosis with aciduria and are involved in the development of glioma. IDH plays a role in the regeneration of NADPH for intraperoxisomal reductions; such as the conversion of 2; 4-dienoyl-CoAs to 3-enoyl-CoAs; as well as in peroxisomal reactions that consume 2-oxoglutarate; namely the α-hydroxylation of phytanic acid.