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Recombinant Human IL-23(IL23A&IL12B Heterodimer) Protein (His Tag)(Active)
SKU: PKSH030445-100
Recombinant Human IL-23(IL23A&IL12B Heterodimer) Protein (His Tag)(Active)
| SKU # | PKSH030445 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CLMF p40, IL-12 subunit p40, IL-23, IL-23A, IL-23p19, IL23P19, NKSF2, P19, SGRF |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Pro189&Met 1-Ser 28 |
| Accession | Q9NPF7&NP_002178.2 |
| Calculated Molecular Weight | 20.1&36.2 kDa |
| Observed Molecular Weight | 22&45 kDa |
| Tag | C-His |
| Bio-activity | 1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. 2. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.28-0.66 μg/ml. 3. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.14-0.35 μg/ml. 4. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 4-20 ng/mL. |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.